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2.\u6750\u6599<\/h2>\n\n\n\n

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1\u3001C57BL\/6J\u96c4\u6027\u5c0f\u9f20\uff0c4-6\u5468\u9f84\uff08\u6765\u81ea\u6770\u514b\u900a\u5b9e\u9a8c\u5ba4\uff09\u3002<\/p>\n\n\n\n

2\u3001\u64cd\u4f5c\u6e05\u5355(DIO\u6a21\u578b) C57BL\/6J\u96c4\u6027\u5c0f\u9f20\uff08Jackson\u5b9e\u9a8c\u5ba4\uff09\uff08\u89c1\u6ce81\uff09\u3002<\/p>\n\n\n\n

\u6ce81. \u6770\u514b\u900a\u5b9e\u9a8c\u5ba4\u63d0\u4f9b\u5404\u79cd\u996e\u98df\uff0c\u53ef\u4ee5\u7528\u6765\u8bf1\u53d1\u80a5\u80d6\u3002\u4f8b\u5982\uff0cC57BL\/6J\u5c0f\u9f20\u53ef\u4ee5\u57286\u5468\u9f84\u65f6\u5f00\u59cb\u5582\u98df60%\u8102\u80aa\u4f9b\u80fd\u7684\u9ad8\u8102\u80aa\u996e\u98df\uff0c\u800c\u5bf9\u7167\u7ec4\u5c0f\u9f20\u53ef\u4ee5\u5582\u98df10%\u8102\u80aa\u4f9b\u80fd\u7684\u6b63\u5e38\u996e\u98df\u3002\u5e74\u9f84\u8303\u56f4\u4e3a6-26\u5468\u3002\u4ed6\u4eec\u5efa\u7acb\u4e86\u751f\u7406\u6570\u636e\u5e93\u548c\u5c0f\u9f20\u6e05\u5355\uff0c\u53ef\u4ee5\u8282\u7701\u5927\u91cf\u7684\u5b9e\u9a8c\u65f6\u95f4\u3002\u5728\u4f7f\u7528\u8fd9\u4e9b\u8001\u9f20\u65f6\uff0c\u5e94\u8be5\u8fdb\u884c\u4e00\u5b9a\u7684\u62a4\u7406\u3002\u9996\u5148\uff0c\u5e94\u8be5\u8003\u8651\u5230\u8fd0\u8f93\u7684\u5f71\u54cd\uff0c\u56e0\u4e3a\u8001\u9f20\u5728\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u4f1a\u4ea7\u751f\u5e94\u6fc0\u9020\u6210\u4f53\u91cd\u4e0b\u964d\u3002\u5728\u4f7f\u7528\u5c0f\u9f20\u4e4b\u524d\uff0c\u6211\u4eec\u901a\u5e38\u5728\u5b9e\u9a8c\u73af\u5883\u4e2d\u9002\u5e941\u5468\u3002\u5176\u6b21\uff0c\u6211\u4eec\u901a\u5e38\u4e0d\u4f1a\u628a\u8fd9\u4e9b\u5c0f\u9f20\u76f8\u4e92\u6df7\u517b\u3002\u96c4\u6027\u8001\u9f20\u503e\u5411\u4e8e\u6253\u67b6\uff0c\u8fd9\u5c06\u5bf9\u5b83\u4eec\u7684\u4f53\u91cd\u4ea7\u751f\u4e00\u4e9b\u5f71\u54cd\u3002<\/p>\n\n\n\n

2.2 \u996e\u98df<\/strong><\/p>\n\n\n\n

1. 10%\u8102\u80aa\u4f9b\u80fd\u996e\u98df\uff08\u6807\u51c6\u996e\u98df\uff09\uff08\u89c1\u6ce82\uff09\u3002<\/p>\n\n\n\n

2. 45%\u8102\u80aa\u4f9b\u80fd\u996e\u98df(\u7814\u7a76\u996e\u98df\uff0cD12451i)\u3002<\/p>\n\n\n\n

3. 60%\u8102\u80aa\u4f9b\u80fd\u996e\u98df\uff08\u7814\u7a76\u996e\u98df\uff0cD12492i\uff09\uff08\u89c1\u6ce83\uff09<\/p>\n\n\n\n

\u6ce82. \u7814\u7a76\u996e\u98dfD12450B\u3002\u5b83\u7684\u70ed\u91cf\u7ec4\u6210\u662f20Kcal%\u7684\u86cb\u767d\u8d28\uff0c70Kcal%\u7684\u78b3\u6c34\u5316\u5408\u7269\u548c10Kcal%\u7684\u8102\u80aa\u3002\u6bcf\u4e00\u514b\u5b83\u7684\u6210\u5206\u542b\u67093.85Kcal\u5361\u8def\u91cc\uff0c\u5176\u4e2d\u5305\u62ec19\u6beb\u514b\u7684\u732a\u6cb9\u80c6\u56fa\u9187\u548c\u9ec4\u8272\u67d3\u6599\u3002<\/p>\n\n\n\n

\u6ce83.\u7814\u7a76\u996e\u98dfD12492\u3002\u5b83\u7684\u70ed\u91cf\u7ec4\u6210\u662f20Kcal%\u7684\u86cb\u767d\u8d28\uff0c20Kcal%\u7684\u78b3\u6c34\u5316\u5408\u7269\u548c60Kcal%\u7684\u8102\u80aa\u3002\u6bcf\u514b\u6210\u5206\u542b\u67095.24Kcal\u5361\u8def\u91cc\uff0c\u5305\u62ec\u732a\u6cb9\u548c\u84dd\u8272\u67d3\u6599\u4e2d232\u6beb\u514b\u7684\u80c6\u56fa\u9187\u3002\u5b83\u5e94\u51b7\u51bb\u50a8\u5b58\uff0c\u53ef\u4ee5\u4fdd\u5b586\u4e2a\u6708\u3002\u5f53\u8003\u8651\u5230\u4eba\u7c7b\u7684\u996e\u98df\u65f6\uff0c60Kcal%\u7684\u8102\u80aa\u5f88\u5c11\u4f1a\u5f88\u5b9e\u7528\u3002\u7136\u800c\uff0c\u4e3a\u4e86\u5728\u66f4\u77ed\u7684\u65f6\u95f4\u5185\u8bf1\u5bfc\u5c0f\u9f20\u80a5\u80d6\u5e76\u4fc3\u8fdb\u7814\u7a76\uff0c\u901a\u5e38\u4f7f\u752860Kcal%\u7684\u9ad8\u8102\u996e\u98df\u3002\u7136\u800c\uff0c\u5f53\u7b5b\u9009\u836f\u7269\u6216\u57fa\u56e0\u64cd\u7eb5\u7684\u5f71\u54cd\u65f6\uff0c\u6709\u65f6\u5b83\u53ef\u80fd\u9700\u898145Kcal%\u7684\u8102\u80aa\u996e\u98df\uff0c\u56e0\u4e3a\u5b83\u53ef\u80fd\u66f4\u96be\u9884\u9632\u6216\u9006\u8f6c\u8fd9\u79cd\u6781\u7aef\u768460Kcal%\u7684\u996e\u98df\u7684\u5f71\u54cd\u3002<\/p>\n\n\n\n

2.3.\u8461\u8404\u7cd6\u548c\u80f0\u5c9b\u7d20\u8bd5\u9a8c<\/strong>\uff08\u4e3a\u6b63\u786e\u8868\u8ff0\uff0c\u5c31\u4e0d\u505a\u7ffb\u8bd1\u4e86\uff09<\/p>\n\n\n\n

1. <\/strong>Dulbecco\u2019s phosphate-buffered saline (D-PBS), 1\u00d7 Liquid (Gibco 14190-136).<\/p>\n\n\n\n

2. <\/strong>NaCl 0.9% sterilized, Baxter IV solutions (Baxter 6E1322).<\/p>\n\n\n\n

3. <\/strong>Disposable sterile animal feeding needles, Popper & Sons, 20 G \u00d7 1 1\/2 in. (VWR 20068-666).<\/p>\n\n\n\n

4. <\/strong>Tuberculin syringe with 27 G \u00d7 1\/2 in. needle (Becton- Dickinson, 309623).<\/p>\n\n\n\n

5. <\/strong>Heated hard pad.<\/p>\n\n\n\n

6. <\/strong>Clean cages with water bottles.<\/p>\n\n\n\n

7. <\/strong>D-(+)-glucose 99.5% (Sigma, G7528).<\/p>\n\n\n\n

8. <\/strong>Human insulin: Novolin R, 100 U\/ml, 10 ml (NovoNordisk).<\/p>\n\n\n\n

9. <\/strong>Scalpel.<\/p>\n\n\n\n

10. <\/strong>Mineral oil (Sigma M3516).<\/p>\n\n\n\n

11. <\/strong>Paper towels.<\/p>\n\n\n\n

12. <\/strong>Ascensia ELITE XL Bayer Glucometer Blood (Bayer).<\/p>\n\n\n\n

13. <\/strong>Ascensia ELITE XL test strips (Bayer).<\/p>\n\n\n\n

14. <\/strong>Batteries, 3 V Lithium cell (Sigma, B0653).<\/p>\n\n\n\n

15. <\/strong>SurePrep\u2122 capillary tubes, 75 mm, self-sealing (Becton Dickinson 420315).<\/p>\n\n\n\n

16. <\/strong>MicroWell 96-well polystyrene plates, round bottom (non-treated), sterile (Nunc, Sigma, P4241).<\/p>\n\n\n\n

17. <\/strong>20\u2013200-\u03bcl pipette with tips.<\/p>\n\n\n\n

18. <\/strong>Insulin (Mouse) Ultrasensitive (Alpco Diagnostics, EIA 80- INSMSU-E10).<\/p>\n\n\n\n

19. <\/strong>Titer Plate Shaker (Thermo Scientific EW-51402-10).<\/p>\n\n\n\n

20. <\/strong>Multilabel Readers, VICTOR (6) (PerkinElmer).<\/p>\n\n\n\n

2.4.\u8840\u6db2\u91c7\u96c6\u548c\u7ec4\u7ec7\u91c7\u96c6<\/strong>\uff08\u4e0d\u7ffb\u8bd1\uff09<\/p>\n\n\n\n

1. <\/strong>1-ml syringes with 25 G \u00d7 5\/8 needle (Becton-Dickinson 305122).<\/p>\n\n\n\n

2. <\/strong>Microtainer serum separator tubes (BD Diagnostics, 365956).<\/p>\n\n\n\n

3. <\/strong>MicroWell 96-well polystyrene plates, round bottom (non-treated), sterile (Nunc, Sigma, P4241).<\/p>\n\n\n\n

4. <\/strong>Formalin solution, neutral buffered 10% (Sigma HT501128).<\/p>\n\n\n\n

5. <\/strong>50-ml conical tubes (VWR 21008-178).<\/p>\n\n\n\n

6. <\/strong>Rodent restrainer 115 VAC, animal weight range 70\u2013125 g (Harvard apparatus).<\/p>\n\n\n\n

3.\u65b9\u6cd5<\/strong><\/h2>\n\n\n\n

\u996e\u98df\u8bf1\u5bfc\u80a5\u80d6\u6a21\u578b\u901a\u5e38\u9700\u898116-20\u5468\u7684\u65f6\u95f4\u5b8c\u6210\u5b9e\u9a8c\uff08\u56fe1\uff09\u3002\u6b64\u5916\uff0c\u80a5\u80d6\u662f\u4e00\u79cd\u591a\u5668\u5b98\u7cfb\u7edf\u75be\u75c5\uff0c\u6d89\u53ca\u4f53\u91cd\u3001\u8102\u80aa\u7ec4\u7ec7\u3001\u8461\u8404\u7cd6\u4ee3\u8c22\u3001\u80c6\u56fa\u9187\u6c34\u5e73\u548c\u708e\u75c7\u6807\u5fd7\u7269\u7684\u7d0a\u4e71\u3002\u56e0\u6b64\uff0c\u5b9e\u9a8c\u524d\u8bbe\u8ba1\u662f\u5b9e\u9a8c\u6210\u529f\u7684\u5173\u952e\u3002\u867d\u7136\u53ef\u4ee5\u6d4b\u8bd5\u591a\u4e2a\u5668\u5b98\u7cfb\u7edf\uff0c\u8bb8\u591a\u53c2\u6570\u53ef\u4ee5\u68c0\u67e5\uff0c\u4f46\u6709\u9884\u5148\u7684\u5b9e\u9a8c\u5047\u8bbe\u548c\u76ee\u6807\u662f\u5f88\u91cd\u8981\u7684\u3002\u5728\u80a5\u80d6\u7684\u53d1\u5c55\u8fc7\u7a0b\u4e2d\uff0c\u4eba\u4eec\u53ef\u4ee5\u68c0\u67e5\u548c\u6d4b\u91cf\u5404\u79cd\u5404\u6837\u7684\u53c2\u6570\u3002\u7136\u800c\uff0c\u4e0d\u540c\u7684\u5047\u8bbe\u9700\u8981\u4e0d\u540c\u7684\u6d4b\u91cf\u548c\u5b9e\u9a8c\u65b9\u6cd5\uff0c\u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\uff0c\u8fd9\u53ef\u80fd\u4f1a\u5f71\u54cd\u751f\u7406\u53c2\u6570\u3002\u5bf9\u8fd9\u4e9b\u5dee\u5f02\u7684\u8be6\u7ec6\u4ecb\u7ecd\u5982\u4e0b\u3002<\/p>\n\n\n\n

\"\"
\u56fe1 a.\u793a\u610f\u56fe\u663e\u793a\u4e86\u6211\u4eec\u5982\u4f55\u8bbe\u8ba1\u9ad8\u8102\u80aa\u548c\u5e38\u89c4\u9972\u6599\u996e\u98df\u5582\u517b\u5728\u6ca1\u6709\u6cbb\u7597\u5e72\u9884\u7684\u72b6\u6001\u4e0b\u3002\u5728\u672c\u5b9e\u9a8c\u4e2d\uff0c\u6211\u4eec\u4f7f\u7528\u96f7\u5e15\u9709\u7d20\uff0c\u6765\u68c0\u6d4b\u96f7\u5e15\u9709\u7d20\u6cbb\u75974\u5468\u662f\u5426\u5bf9\u4f53\u91cd\u6709\u5f71\u54cd\u3002b.\u5c0f\u9f20\u5582\u98df\u9ad8\u8102\u80aa\u996e\u98df\u6216\u9972\u6599\u65f6\u7684\u5178\u578b\u4f53\u91cd\u56fe(Adapted from Wang et al. Science Signaling (2009) withpermission)<\/figcaption><\/figure>\n\n\n\n

3.1 \u9972\u517b\u73af\u5883<\/strong><\/p>\n\n\n\n

1.\u5c06\u5c0f\u9f20\u4fdd\u6301\u5728\u6807\u51c6\u7684\u65e0\u75c5\u539f\u4f53\u6761\u4ef6\uff08SPF\u7ea7\uff09\u4e0b\uff0c\u81ea\u7531\u98df\u7528\u98df\u7269\u548c\u6c34\uff0c\u5e76\u6709\u89c4\u5f8b\u768412\uff1a12\u5149-\u6697\u5faa\u73af\u3002\u786e\u4fdd\u4e25\u683c\u9075\u5faa\u5149-\u6697\u5468\u671f\uff0c\u56e0\u4e3a\u5728\u6697\u5468\u671f\u5f00\u59cb\u540e\u68c0\u67e5\u5c0f\u9f20\u4f1a\u6270\u4e71\u5c0f\u9f20\u7684\u6b63\u5e38\u663c\u591c\u8282\u5f8b\uff0c\u5e76\u5f71\u54cd\u5c0f\u9f20\u7684\u4ee3\u8c22\u3002<\/p>\n\n\n\n

2.\u6bcf\u4e2a\u7b3c\u5b50\u91cc\u90fd\u5e94\u5b89\u7f6e\u76f8\u540c\u6570\u91cf\u7684\u8001\u9f20\uff08\u89c1\u6ce84\uff09\u3002<\/p>\n\n\n\n

3.\u6bcf\u5929\u68c0\u67e5\u7b3c\u5b50\u548c\u57ab\u6599\u7684\u60c5\u51b5\uff0c\u4f46\u907f\u514d\u68c0\u67e5\u8001\u9f20\u592a\u9891\u7e41\u3002C57BL\/6J\u5c0f\u9f20\u867d\u7136\u4e0d\u50cf\u5176\u4ed6\u8f6c\u57fa\u56e0\u7cd6\u5c3f\u75c5\u5c0f\u9f20\u90a3\u6837\u9700\u8981\u9891\u7e41\u66f4\u6362\u70b9\u6765\u54e6\uff0c\u4f46\u5728\u80a5\u80d6\u548c\u7cd6\u5c3f\u75c5\u540e\u4f1a\u51fa\u73b0\u591a\u5c3f\uff0c\u5c0f\u9f20\u7b3c\u5b50\u53ef\u80fd\u4e5f\u9700\u8981\u66f4\u9891\u7e41\u5730\u66f4\u6362\u57ab\u6599\u3002<\/p>\n\n\n\n

4.\u5728\u5b9e\u9a8c\u5f00\u59cb\u65f6\u7528\u8033\u6807\u6216\u5bf9\u5c0f\u9f20\u8fdb\u884c\u6807\u8bb0\uff0c\u65b9\u4fbf\u8bc6\u522b\uff0c\u56e0\u4e3a\u8fd9\u4e9b\u7a0b\u5e8f\u4e5f\u4f1a\u5f71\u54cd\u5c0f\u9f20\u7684\u4f53\u91cd\u548c\u8461\u8404\u7cd6\u3002<\/p>\n\n\n\n

5.\u6ce8\u610f\u9f20\u7b3c\u9644\u8fd1\u7684\u73af\u5883\u3002\u566a\u97f3\u3001\u4ea4\u901a\u6216\u5176\u4ed6\u5e72\u6270\u4f1a\u5f71\u54cd\u4f53\u91cd\uff0c\u5e76\u53ef\u80fd\u4f1a\u5bf9\u996e\u98df\u8bf1\u5bfc\u7684\u80a5\u80d6\u5b9e\u9a8c\u9020\u6210\u504f\u5dee\u3002<\/p>\n\n\n\n

\u6ce84. \u548c\u8bb8\u591a\u54fa\u4e73\u52a8\u7269\u4e00\u6837\uff0c\u7b3c\u5b50\u91cc\u7684C57BL\/6J\u5c0f\u9f20\u4e5f\u5efa\u7acb\u4e86\u793e\u4f1a\u7b49\u7ea7\u5236\u5ea6\uff0c\u51b3\u5b9a\u4e86\u98df\u7269\u3001\u6c34\u3001\u4f11\u606f\u5730\u70b9\u548c\u4ea4\u914d\u7684\u5206\u914d\u3002\u7b3c\u5b50\u91cc\u4e0d\u540c\u7684\u8001\u9f20\u6570\u91cf\u4f1a\u5bfc\u81f4\u4e0d\u540c\u7684\u793e\u4f1a\u884c\u4e3a\uff0c\u5e76\u4f1a\u5bf9\u80a5\u80d6\u7684\u53d1\u5c55\u4ea7\u751f\u5f71\u54cd\u3002<\/p>\n\n\n\n

3.2.\u5c0f\u9f20\u7684\u968f\u673a\u5316\u548c\u5b9e\u9a8c\u8bbe\u8ba1<\/strong><\/p>\n\n\n\n

\u7531\u4e8e\u5373\u4f7f\u5728\u5c0f\u9f20\u7684\u8fd1\u4ea4\u7cfb\u4e2d\u4e5f\u5b58\u5728\u4e2a\u4f53\u5dee\u5f02\uff0c\u56e0\u6b64\u5728\u5b9e\u9a8c\u524d\u6536\u96c6\u5c0f\u9f20\u7684\u57fa\u7ebf\u6570\u636e\u662f\u5f88\u91cd\u8981\u7684\u3002\u6211\u4eec\u6d4b\u91cf\u4e86\u540c\u6027\u522b\u548c\u540c\u5e74\u9f84\u7684\u5c0f\u9f20\u7684\u4f53\u91cd\u3001\u80eb\u9aa8\u957f\u5ea6\u3001\u7a7a\u8179\u8840\u7cd6\u3002\u5728\u6211\u4eec\u7684\u5b9e\u9a8c\u5ba4\u4e2d\uff0cC57BL\/6J\u96c4\u6027\u5c0f\u9f20\u7684\u57fa\u7ebf\u7a7a\u8179\u8840\u7cd6\u8303\u56f4\u4e3a40-190mg\/dl\uff1b\u6211\u4eec\u53ea\u91c7\u7528\u4e86\u7a7a\u8179\u8840\u7cd6\u572870-130mg\/dl\u4e4b\u95f4\u7684\u5c0f\u9f20\u3002\u6211\u4eec\u8fd8\u6392\u9664\u4e866\u5468\u9f84\u65f6\u4f53\u91cd\u4f4e\u4e8e15\u514b\u6216\u8d85\u8fc725\u514b\u7684\u96c4\u6027\u5c0f\u9f20\u3002\u957f\u76f8\u4e0d\u4f73\u3001\u7259\u9f7f\u95ee\u9898\u6216\u6bdb\u53d1\u75be\u75c5\u7684\u5c0f\u9f20\u4e5f\u88ab\u6392\u9664\u5728\u7814\u7a76\u4e4b\u5916\u3002<\/p>\n\n\n\n

1.\u57285\u5468\u9f84\u65f6\uff0c\u6d4b\u91cf\u5c0f\u9f20\u7684\u4f53\u91cd\u548c\u80eb\u9aa8\u957f\u5ea6\u3002<\/p>\n\n\n\n

2.\u6d4b\u91cf\u57fa\u7ebf\u8840\u7cd6\uff1a\u5c06\u8840\u7cd6\u4eea\u6761\u63d2\u5165\u8840\u7cd6\u4eea\u3002\u5e26\u6709\u624b\u5957\u7684\u624b\u6293\u53d6\u8001\u9f20\uff0c\u7528\u53e6\u4e00\u53ea\u624b\u6216\u8001\u9f20\u7ea6\u675f\u5668\u7ea6\u675f\u8001\u9f20\u3002\u6211\u4eec\u901a\u5e38\u4f7f\u7528\u975e\u52a0\u70ed\u7248\u672c\u7684\u556e\u9f7f\u52a8\u7269\u7ea6\u675f\u5668\u3002\u5b83\u662f\u5706\u67f1\u5f62\u7684\uff0c\u6bcf\u4e00\u9762\u90fd\u6709\u4e00\u7cfb\u5217\u7684\u5b54\uff0c\u65b9\u4fbf\u6ce8\u5c04\u6216\u9a8c\u8840\u3002<\/p>\n\n\n\n

3.\u6309\u4f53\u91cd\u968f\u673a\u5206\u7ec4\u5c0f\u9f20\uff0c\u5e76\u6392\u9664\u4f53\u91cd\u6781\u7aef\u7684\u5c0f\u9f20\u3002<\/p>\n\n\n\n

4.\u5217\u51fa\u5404\u5b9e\u9a8c\u7ec4\u7684\u8840\u7cd6\u6c34\u5e73\u3002<\/p>\n\n\n\n

5.\u6392\u9664\u8461\u8404\u7cd6\u6c34\u5e73\u6781\u9ad8\u7684\u5c0f\u9f20\u3002<\/p>\n\n\n\n

6.\u6267\u884c\u7edf\u8ba1\u91cf\u68c0\u67e5\uff0c\u4ee5\u786e\u4fdd\u4e0d\u5b58\u5728\u5206\u7ec4\u504f\u5dee\u3002<\/p>\n\n\n\n

3.3.\u9ad8\u8102\u996e\u98df\u5582\u517b\u53ca\u98df\u7269\u6444\u5165\u91cf\u7684\u6d4b\u91cf<\/strong><\/p>\n\n\n\n

1.\u9009\u62e9\u4e0d\u540c\u7684\u996e\u98df\u65b9\u6848\u3002\u4f8b\u5982\uff0c\u5bf9\u4e8eC57BL\/6J\u996e\u98df\u8bf1\u5bfc\u7684\u80a5\u80d6\u6a21\u578b\uff0c\u6211\u4eec\u901a\u5e38\u5bf9\u5b9e\u9a8c\u7ec4\u4f7f\u752860Kcal%\u8102\u80aa\u996e\u98df\uff0c\u5bf9\u7167\u7ec4\u4f7f\u752810Kcal%\u8102\u80aa\u996e\u98df\uff0c\u8fd9\u53d6\u51b3\u4e8e\u5b9e\u9a8c\u7684\u76ee\u7684\uff08\u89c1\u6ce85\uff09\u3002<\/p>\n\n\n\n

2.\u5728\u6539\u53d8\u996e\u98df\u4e4b\u524d\u6d4b\u91cf\u4f53\u91cd\u3002\u9009\u62e9\u6709\u9897\u7c92\u5b8c\u6574\u5ea6\u597d\u7684\u996e\u98df\uff0c\u6d88\u9664\u7834\u788e\u7684\u98df\u7269\u9897\u7c92\u6216\u7c89\u672b\u3002<\/p>\n\n\n\n

3.\u628a\u98df\u7269\u5012\u8fdb\u7b3c\u5b50\u91cc\uff0c\u907f\u514d\u628a\u788e\u7247\u6216\u7c89\u672b\u5012\u8fdb\u7b3c\u5b50\u91cc\uff0c\u56e0\u4e3a\u5b83\u4eec\u6709\u65f6\u4f1a\u548c\u591a\u5c3f\u5c0f\u9f20\u7684\u5c3f\u6db2\u4e00\u8d77\u6c61\u67d3\u57ab\u6599\uff0c\u800c\u4e14\u8fd9\u4e9b\u788e\u7247\u6216\u7c89\u672b\u4e5f\u4f1a\u5bfc\u81f4\u98df\u7269\u6444\u5165\u91cf\u6d4b\u91cf\u7684\u8bef\u5dee\u3002<\/p>\n\n\n\n

4.\u6bcf\u5929\u89c2\u5bdf\u98df\u7269\u7684\u989c\u8272\u3002\u8fd9\u4e9b60%\u8102\u80aa\u4f9b\u80fd\u7684\u9ad8\u8102\u80aa\u996e\u98df\u66b4\u9732\u5728\u7a7a\u6c14\u4e2d2-3\u5929\u540e\u5f80\u5f80\u4f1a\u53d8\u6210\u53d8\u8f6f\u6216\u8005\u53d8\u5473\u3002<\/p>\n\n\n\n

5.\u6bcf\u4e24\u5929\u66f4\u6362\u996e\u98df\uff0c\u6d4b\u91cf\u98df\u7269\u91cd\u91cf\u7684\u53d8\u5316\uff0c\u4ee5\u6d4b\u91cf\u5c0f\u9f20\u7684\u98df\u7269\u6444\u5165\u91cf\u3002\u6d4b\u91cf\u524d\uff0c\u5c3d\u53ef\u80fd\u53d6\u51fa\u57ab\u6599\u4e0a\u7684\u9972\u6599\u9897\u7c92\uff0c\u4ee5\u589e\u52a0\u6d4b\u7b97\u7684\u7cbe\u5ea6\u3002<\/p>\n\n\n\n

6.\u901a\u5e38\u4f1a\u6839\u636e\u9700\u8981\u653e\u66f4\u591a\u7684\u98df\u7269\u3002\u6211\u4eec\u4e0d\u5728\u6bcf\u6b21\u6d4b\u91cf\u4e4b\u95f4\u6dfb\u52a0\u65b0\u9c9c\u98df\u7269\uff0c\u56e0\u4e3a\u8fd9\u53ef\u80fd\u5bfc\u81f4\u7ec4\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u968f\u7740\u5b9e\u9a8c\u7684\u7ee7\u7eed\uff0c\u4f53\u91cd\u7684\u589e\u957f\uff0c\u9ad8\u8102\u80aa\u996e\u98df\u7ec4\u901a\u5e38\u4f1a\u6bd4\u5bf9\u7167\u7ec4\u6709\u66f4\u591a\u7684\u80fd\u91cf\u6444\u5165\u91cf\u3002<\/p>\n\n\n\n

7.\u5f53\u80a5\u80d6\u5c0f\u9f20\u51fa\u73b0\u591a\u5c3f\u65f6\uff0c\u53ef\u80fd\u9700\u8981\u6bcf\u5468\u66f4\u6362\u57ab\u6599\u8d85\u8fc7\u4e24\u5230\u4e09\u6b21\u3002\u786e\u4fdd\u66f4\u6362\u57ab\u6599\u4e0d\u4f1a\u5728\u6d4b\u91cf\u98df\u7269\u6444\u5165\u91cf\u65f6\u9020\u6210\u9519\u8bef\u3002<\/p>\n\n\n\n

8.\u5728\u66f4\u6362\u57ab\u6599\u7684\u540c\u65f6\uff0c\u540c\u65f6\u8fdb\u884c\u4f53\u91cd\u6d4b\u91cf\uff08\u89c1\u6ce86\uff09<\/p>\n\n\n\n

\u6ce85 \u6765\u81ea\u4e0d\u540c\u4f9b\u5e94\u5546\u7684\u5e38\u89c4\u98df\u7269\u53ef\u80fd\u6709\u4e0d\u540c\u7684\u6210\u5206\u3002\u5361\u8def\u91cc\u7684\u7ec4\u6210\u901a\u5e38\u662f\u86cb\u767d\u8d2820%\uff0c\u8102\u80aa10%\uff0c\u78b3\u6c34\u5316\u5408\u726970%\u3002\u7136\u800c\uff0c\u5b83\u4eec\u7684\u80c6\u56fa\u9187\u3001\u7ef4\u751f\u7d20\u3001\u7ea4\u7ef4\u548c\u8102\u80aa\u6210\u5206\u53ef\u80fd\u4f1a\u6709\u6240\u4e0d\u540c\u3002\u4e0d\u540c\u5b9e\u9a8c\u4e4b\u95f4\u7684\u4e00\u81f4\u6027\u662f\u5f88\u91cd\u8981\u7684\uff0c\u6240\u4ee5\u9700\u8981\u5c3d\u53ef\u80fd\u7684\u4e0e\u5b9e\u9a8c\u76ee\u7684\u4fdd\u6301\u4e00\u81f4\u9009\u62e9\u98df\u7269\u3002<\/p>\n\n\n\n

\u6ce86 \u8001\u9f20\u662f\u793e\u4f1a\u6027\u52a8\u7269\u3002\u901a\u5e38\u6709\u4e00\u4e9b\u8001\u9f20\u5728\u7b3c\u5b50\u91cc\u5360\u4e3b\u5bfc\u5730\u4f4d\uff0c\u800c\u6709\u4e9b\u5219\u4e0d\u662f\u3002\u5728\u6d4b\u91cf\u4f53\u91cd\u65f6\uff0c\u6211\u4eec\u901a\u5e38\u4f1a\u6807\u8bb0\u54ea\u4e9b\u8001\u9f20\u5360\u4e3b\u5bfc\u5730\u4f4d\uff0c\u54ea\u4e9b\u4e0d\u662f\uff0c\u5e76\u5728\u4e24\u7ec4\u4e4b\u95f4\u8fdb\u884c\u6bd4\u8f83\uff0c\u4ee5\u4fbf\u66f4\u597d\u5730\u4e86\u89e3\u5b83\u4eec\u7684\u4f53\u91cd\u53d8\u5316\u3002<\/p>\n\n\n\n

3.4.\u5c3e\u90e8\u53d6\u8840\u6d4b\u91cf<\/strong><\/p>\n\n\n\n

1.\u5c06\u8840\u7cd6\u4eea\u5e26\u63d2\u5165\u8840\u7cd6\u4eea\u3002<\/p>\n\n\n\n

2.\u5e26\u6709\u624b\u5957\u7684\u624b\u6293\u53d6\u8001\u9f20\u3002\u7528\u53e6\u4e00\u53ea\u624b\u6216\u8001\u9f20\u7ea6\u675f\u5668\u7ea6\u675f\u8001\u9f20\u3002\u6211\u4eec\u901a\u5e38\u4f7f\u7528\u975e\u52a0\u70ed\u7248\u672c\u7684\u556e\u9f7f\u52a8\u7269\u7ea6\u675f\u5242\u3002\u5b83\u5448\u5706\u67f1\u5f62\uff0c\u6bcf\u4e00\u8fb9\u90fd\u6709\u4e00\u7cfb\u5217\u7684\u5b54\uff0c\u4fbf\u4e8e\u6ce8\u5c04\u6216\u8840\u6db2\u6d4b\u8bd5\u3002\u5982\u679c\u6709\u7ecf\u9a8c\uff0c\u89d2\u5ea6\u578b\u7ea6\u675f\u5668\u4f1a\u66f4\u7b80\u5355\u3002<\/p>\n\n\n\n

3.\u7528\u624b\u672f\u5200\u5c3d\u53ef\u80fd\u5c11\u5730\u5272\u65ad\u5c3e\u5df4\u3002<\/p>\n\n\n\n

4.\u628a\u5c3e\u90e8\u7684\u4e00\u6ef4\u8840\u653e\u5728\u8840\u7cd6\u4eea\u6761\u4e0a\u5e76\u8bfb\u53d6\u3002<\/p>\n\n\n\n

5.\u5bf9\u4e8e\u8840\u6e05\u6216\u9664\u8461\u8404\u7cd6\u4ee5\u5916\u7684\u66f4\u591a\u7684\u8840\u6db2\uff0c\u4f7f\u7528\u6bdb\u7ec6\u7ba1\u3002\u5982\u679c\u5728\u6bdb\u7ec6\u7ba1\u91c7\u96c6\u8fc7\u7a0b\u4e2d\u8840\u6db2\u505c\u6b62\uff0c\u8bf7\u5411\u5c16\u7aef\u6309\u6469\u5c3e\u5df4\u3002<\/p>\n\n\n\n

6.\u5982\u679c\u9700\u8981\u66f4\u591a\u7684\u8840\u6db2\uff0c\u8bf7\u4ece\u7736\u4e0b\u533a\u57df\u91c7\u96c6\u8840\u6db2\u3002<\/p>\n\n\n\n

3.5.\u8461\u8404\u7cd6\u8010\u91cf\u8bd5\u9a8c<\/strong><\/p>\n\n\n\n

\u51c6\u786e\u7684\u8461\u8404\u7cd6\u8010\u91cf\u6d4b\u8bd5\u53d6\u51b3\u4e8e\u5c0f\u9f20\u7684\u5236\u5907\u3002 \u4e3a\u4e86\u5bc6\u5207\u6a21\u62df\u8461\u8404\u7cd6\u8010\u91cf\u6d4b\u8bd5\u7684\u4eba\u7c7b\u90e8\u5206\uff0c\u6211\u4eec\u5728\u4e0a\u5348 7:00 \u5230\u4e0b\u5348 15:00 \u5bf9\u5c0f\u9f20\u8fdb\u884c\u7981\u98df\u3002 \u5c0f\u9f20\u5728\u660e\u6697\u9636\u6bb5\u662f\u6d3b\u8dc3\u7684\uff0c\u4e0e\u4eba\u7c7b\u76f8\u6bd4\uff0c\u8fd9\u4e2a\u7981\u98df\u671f\u66f4\u5177\u751f\u7406\u6027\u3002 \u5982\u679c\u6ca1\u6709\u9002\u5f53\u7684\u7981\u98df\uff0c\u8010\u53d7\u8bd5\u9a8c\u540e\u7684\u57fa\u7ebf\u8461\u8404\u7cd6\u8bfb\u6570\u548c\u8461\u8404\u7cd6\u6c34\u5e73\u5c06\u96be\u4ee5\u89e3\u91ca\u3002\u5728\u6211\u4eec\u7684\u5b9e\u9a8c\u5ba4\uff0c\u6211\u4eec\u901a\u5e38\u901a\u8fc7\u8179\u8154\u6ce8\u5c04\u8461\u8404\u7cd6\u3002 \u6ce8\u5c04\u66f4\u7b26\u5408\u5242\u91cf\u548c\u7ed3\u679c\u3002 \u5982\u679c\u8981\u6d4b\u8bd5\u8bb8\u591a\u8001\u9f20\uff0c\u5b83\u4e5f\u53ef\u4ee5\u8282\u7701\u65f6\u95f4\u3002 \u4f46\u662f\uff0c\u5b83\u4e0d\u662f\u751f\u7406\u6027\u7684\uff0c\u4e0d\u80fd\u5b8c\u5168\u4e0e\u4eba\u4f53\u8461\u8404\u7cd6\u8010\u91cf\u8bd5\u9a8c\u76f8\u6bd4\u8f83\u800c\u5ffd\u7565\u8461\u8404\u7cd6\u5bf9\u80c3\u80a0\u9053\u7684\u5f71\u54cd\u3002<\/p>\n\n\n\n

1\u3001\u6e05\u7406\u7b3c\u5177\u5185\u7684\u57ab\u6599\u548c\u98df\u7269\u3002 \u786e\u4fdd\u57ab\u6599\u4e2d\u6ca1\u6709\u98df\u7269\u3002 \u4fdd\u7559\u6c34\u74f6\u3002<\/p>\n\n\n\n

2\u3001\u7ed9\u8001\u9f20\u79f0\u91cd\u3002<\/p>\n\n\n\n

3\u3001\u5728 PBS \u4e2d\u5236\u5907\u65e0\u83cc 10% (w\/v) \u8461\u8404\u7cd6\u6eb6\u6db2\u3002 \u8461\u8404\u7cd6\u5c06\u5728 10 ml\/kg \u4f53\u79ef\u4e2d\u4ee5 1 g\/kg\uff08\u8461\u8404\u7cd6\/\u4f53\u91cd\uff09\u7684\u6d53\u5ea6\u7ed9\u836f\u3002\u6709\u65f6\uff0c\u6211\u4eec\u4f1a\u5728\u5c0f\u9f20\u60a3\u7cd6\u5c3f\u75c5\u4e4b\u524d\u7684\u5b9e\u9a8c\u65e9\u671f\u9009\u62e9 2 g\/kg \u6d53\u5ea6\u3002 \u5982\u679c\u5c0f\u9f20\u60a3\u4e0a\u7cd6\u5c3f\u75c5\uff0c2 g\/kg \u6d53\u5ea6\u5c06\u5bfc\u81f4\u8840\u7cd6\u4eea\u4e2d\u7684\u8461\u8404\u7cd6\u503c\u65e0\u6cd5\u8bfb\u53d6\u3002\u7528\u8461\u8404\u7cd6\u6eb6\u6db2\u9884\u88c5 1 ml \u80f0\u5c9b\u7d20\u6ce8\u5c04\u5668\u3002 \u53bb\u9664\u6ce8\u5c04\u5668\u4e2d\u7684\u7a7a\u6c14\u3002<\/p>\n\n\n\n

4\u3001\u7981\u98df\u671f\u7ed3\u675f\u540e\u3002 \u6d4b\u91cf\u57fa\u7ebf\u8461\u8404\u7cd6\u6c34\u5e73\u3002 \u7528\u6234\u624b\u5957\u7684\u624b\u6293\u53d6\u8001\u9f20\u3002 \u7528\u53e6\u4e00\u53ea\u624b\u6216\u8001\u9f20\u7ea6\u675f\u5668\u7ea6\u675f\u8001\u9f20\u3002 \u7528\u624b\u672f\u5200\u5c3d\u91cf\u5c11\u5207\u5c3e\u5df4\u3002 \u5c06\u4e00\u6ef4\u8840\u6db2\u4ece\u5c3e\u90e8\u6ef4\u5230\u8840\u7cd6\u4eea\u6761\u4e0a\u5e76\u8bfb\u53d6\u3002 \u8bb0\u5f55\u8461\u8404\u7cd6\u503c\u3002 \u8461\u8404\u7cd6\u503c\u7684\u8303\u56f4\u662f 20\u2013600 mg\/dl\u3002 \u5982\u679c\u8840\u7cd6\u4eea\u663e\u793a\u503c\u201cHi\u201d\uff0c\u6211\u4eec\u901a\u5e38\u7528\u6bdb\u7ec6\u7ba1\u53d6\u8840\u5e76\u7528 PBS \u7a00\u91ca 2 \u500d\u3002 \u5b9e\u9645\u8461\u8404\u7cd6\u6c34\u5e73\u5f88\u5c11\u8fbe\u5230 1,200 mg\/dl\uff0c\u7a00\u91ca 2 \u500d\u901a\u5e38\u8db3\u4ee5\u663e\u793a\u51c6\u786e\u6c34\u5e73\u3002<\/p>\n\n\n\n

5\u3001\u5145\u5206\u7981\u98df\u540e\uff0c\u7ed9\u5c0f\u9f20\u8179\u8154\u6ce8\u5c04\u8461\u8404\u7cd6\u3002 \u5982\u679c\u5c0f\u9f20\u8f83\u591a\uff0c\u5728 15 \u5206\u949f\u5185\u65e0\u6cd5\u5b8c\u6210\u6ce8\u5c04\uff0c\u6211\u4eec\u901a\u5e38\u4f1a\u4e0e\u4e24\u5230\u4e09\u4e2a\u4eba\u4e00\u8d77\u5de5\u4f5c\uff0c\u4ee5\u907f\u514d\u5ef6\u957f\u6ce8\u5c04\u65f6\u95f4\u3002<\/p>\n\n\n\n

6\u3001\u5206\u522b\u5728 15\u300130\u300160 \u548c 90 \u5206\u949f\u91cd\u590d\u8840\u7cd6\u6d4b\u91cf\u3002<\/p>\n\n\n\n

7\u3001\u6d4b\u91cf\u7ed3\u675f\u540e\uff0c\u5c06\u8001\u9f20\u653e\u56de\u7b3c\u5b50\u91cc\u5e76\u6295\u653e\u98df\u7269\u3002<\/p>\n\n\n\n

3.6.\u80f0\u5c9b\u7d20\u6c34\u5e73\u6d4b\u91cf<\/strong><\/p>\n\n\n\n

\u8840\u6e05\u91c7\u96c6\u540e-20\u2103\u4fdd\u5b58\u3002\u6837\u54c1\u7684\u51bb\u878d\u30014\u2103\u4fdd\u5b58\u6216\u65e0\u6cd5\u4fdd\u8bc1\u8bd5\u5242\u76d2\u8fbe\u5230\u5ba4\u6e29\u5c06\u5bfc\u81f4\u5b9e\u9a8c\u8bef\u5dee\u3002<\/p>\n\n\n\n

1\u3001\u51c6\u5907\u8bd5\u5242\u3002 \u7528 10 \u4efd\u5076\u8054\u7f13\u51b2\u6db2\u7a00\u91ca\u5076\u8054\u7269\u3002\u7528 0.6 ml \u84b8\u998f\u6c34\u91cd\u65b0\u914d\u5236\u80f0\u5c9b\u7d20\u5bf9\u7167\u3002 \u7528 400 ml \u84b8\u998f\u6c34\u7a00\u91ca 20 ml \u6d17\u6da4\u7f13\u51b2\u6db2\u3002<\/p>\n\n\n\n

2\u3001\u786e\u4fdd\u5fae\u5b54\u677f\u5728\u6253\u5f00\u94dd\u7b94\u888b\u4e4b\u524d\u5904\u4e8e\u5ba4\u6e29\u3002\u4e3a\u6807\u51c6\u3001\u5bf9\u7167\u548c\u5c0f\u9f20\u6837\u54c1\u6307\u5b9a\u5fae\u5b54\u677f\u6761\u3002<\/p>\n\n\n\n

3\u3001\u5c06\u6807\u51c6\u54c1\u3001\u5bf9\u7167\u54c1\u6216\u5c0f\u9f20\u6837\u54c1\u540410 \u03bcl \u52a0\u5165\u5176\u5404\u81ea\u7684\u5b54\u4e2d\u3002<\/p>\n\n\n\n

4. \u6bcf\u5b54\u52a0\u5165 75 \u03bcl \u9176\u7ed3\u5408\u7269\uff0c\u7528\u63d0\u4f9b\u7684\u8584\u819c\u5bc6\u5c01\u76d8\u5b50\u3002<\/p>\n\n\n\n

5. \u5c06\u5fae\u5b54\u677f\u7f6e\u4e8e\u5ba4\u6e29\u4e0b\uff0c\u4ee5 700-900 rpm \u7684\u901f\u5ea6\u6447\u52a8 2 \u5c0f\u65f6\u3002<\/p>\n\n\n\n

6. \u7528\u6d17\u6da4\u7f13\u51b2\u6db2\u6d17\u6da4\u5fae\u5b54\u677f\u516d\u6b21\uff0c\u7528\u6d17\u74f6\u8fdb\u884c\u6700\u540e\u4e00\u6b21\u6e05\u6d17\u540e\uff0c\u5c06\u5fae\u5b54\u677f\u5012\u7f6e\u5e76\u7528\u529b\u6572\u51fb\u5438\u6c34\u7eb8\u5dfe\uff0c\u4ee5\u53bb\u9664\u5b54\u4e2d\u4efb\u4f55\u6b8b\u7559\u7684\u7f13\u51b2\u6db2\u548c\u6c14\u6ce1<\/p>\n\n\n\n

7\u3001\u6bcf\u5b54\u52a0\u5165 100 \u03bcl \u5e95\u7269\u30028. \u5728\u5ba4\u6e29\u4e0b\u5728\u6c34\u5e73\u5fae\u5b54\u677f\u632f\u8361\u5668\u4e0a\u5b75\u80b2 15 \u5206\u949f\u3002<\/p>\n\n\n\n

9. \u5c06 100 \u03bcl \u7ec8\u6b62\u6eb6\u6db2\u79fb\u5165\u6bcf\u4e2a\u5b54\u4e2d\u3002 \u8f7b\u8f7b\u6447\u52a8\u5fae\u5b54\u677f\u4ee5\u505c\u6b62\u53cd\u5e94\u3002<\/p>\n\n\n\n

10. \u7528 620\u2013650 nm \u7684\u53c2\u8003\u6ce2\u957f\u8bfb\u53d6 450 nm \u5904\u7684\u5438\u5149\u5ea6\u3002 \u6dfb\u52a0\u7ec8\u6b62\u6eb6\u6db2\u540e 30 \u5206\u949f\u5185\u8bfb\u53d6\u677f\u3002<\/p>\n\n\n\n

3.7. \u8461\u8404\u7cd6\u523a\u6fc0\u7684\u80f0\u5c9b\u7d20\u5206\u6ccc<\/strong><\/p>\n\n\n\n

\u6211\u4eec\u901a\u5e38\u5355\u72ec\u8fdb\u884c\u8461\u8404\u7cd6\u523a\u6fc0\u7684\u80f0\u5c9b\u7d20\u5206\u6ccc\u8bd5\u9a8c\u548c\u8461\u8404\u7cd6\u8010\u91cf\u8bd5\u9a8c\u3002\u4f46\u662f\uff0c\u8461\u8404\u7cd6\u523a\u6fc0\u80f0\u5c9b\u7d20\u5206\u6ccc\u8bd5\u9a8c\u6bd4\u8f83\u7e41\u7410\uff0c\u9700\u8981\u81f3\u5c11\u4e24\u4e2a\u4eba\u540c\u65f6\u8fdb\u884c\u8461\u8404\u7cd6\u8010\u91cf\u8bd5\u9a8c\u548c\u8461\u8404\u7cd6\u523a\u6fc0\u80f0\u5c9b\u7d20\u5206\u6ccc\u8bd5\u9a8c\u3002\u8461\u8404\u7cd6\u8179\u8154\u6ce8\u5c04\u540e\u7684\u5cf0\u503c\u80f0\u5c9b\u7d20\u5206\u6ccc\u7ea6\u4e3a 9-10 \u5206\u949f\u3002\u6211\u4eec\u901a\u5e38\u5728\u8461\u8404\u7cd6\u6ce8\u5c04\u540e 0\u30015\u300110\u300115 \u548c 30 \u5206\u949f\u6536\u96c6\u6837\u672c\u3002\u8461\u8404\u7cd6\u523a\u6fc0\u7684\u80f0\u5c9b\u7d20\u5206\u6ccc\u8bd5\u9a8c\u635f\u4f24\u662f \u03b2 \u7ec6\u80de\u8870\u7aed\u7684\u5173\u952e\u56e0\u7d20\uff0c\u4e00\u4e9b\u57fa\u56e0\u64cd\u4f5c\u5c0f\u9f20\u53ef\u80fd\u4ec5\u5728\u8be5\u8bd5\u9a8c\u4e2d\u663e\u793a\u8868\u578b\uff0c\u800c\u4e0d\u662f\u8461\u8404\u7cd6\u8010\u91cf\u8bd5\u9a8c\u3002<\/p>\n\n\n\n

1\u3001\u5236\u5907\u8461\u8404\u7cd6\u5e76\u6d4b\u91cf\u5c0f\u9f20\u4f53\u91cd\u3002<\/p>\n\n\n\n

2\u3001\u5c06\u57fa\u7ebf\u8840\u6db2\u6536\u96c6\u5230\u6bdb\u7ec6\u7ba1\u4e2d\u3002<\/p>\n\n\n\n

3\u3001\u7ed9\u5c0f\u9f20\u6ce8\u5c04\u8461\u8404\u7cd6\uff081 mg\/kg\uff0c10 ml\/kg \u4f53\u79ef\uff09\u3002<\/p>\n\n\n\n

4\u3001\u8461\u8404\u7cd6\u6ce8\u5c04\u540e 5\u300110\u300115 \u548c 30 \u5206\u949f\u91c7\u8840\u3002<\/p>\n\n\n\n

5\u3001\u5c06\u6bdb\u7ec6\u7ba1\u7f6e\u4e8e\u5fae\u91cf\u8840\u7ec6\u80de\u6bd4\u5bb9\u79bb\u5fc3\u673a\u4e2d\u4ee5 12,000 g \u79bb\u5fc3 10 \u5206\u949f\u3002\u5728\u8840\u6e05\u548c\u7ea2\u7ec6\u80de\u4e4b\u95f4\u7684\u6c34\u5e73\u5904\u5207\u5272\u6bdb\u7ec6\u7ba1\u3002<\/p>\n\n\n\n

6\u3001\u5c06\u8840\u6e05\u52a0\u5165\u5fae\u5b54\u677f\u4e2d\u3002\u6837\u54c1\u53ef\u4ee5\u5728 \u201320\u00b0C \u4e0b\u51b7\u51bb\u4ee5\u4f9b\u4ee5\u540e\u6d4b\u91cf\u3002<\/p>\n\n\n\n

7\u3001\u5982\u524d\u6240\u8ff0\u6d4b\u91cf\u80f0\u5c9b\u7d20\u6c34\u5e73<\/p>\n\n\n\n

3.8. \u80f0\u5c9b\u7d20\u8010\u53d7\u6027\u6d4b\u8bd5<\/strong><\/p>\n\n\n\n

\u80f0\u5c9b\u7d20\u8010\u91cf\u8bd5\u9a8c\u7684\u5242\u91cf\u53ef\u80fd\u4ece 1 \u5230 5 U\/kg \u4e0d\u7b49\uff0c\u5e94\u8003\u8651\u5230\u5c0f\u9f20\u7684\u4f53\u91cd\u3001\u7cd6\u5c3f\u75c5\u72b6\u51b5\u548c\u9057\u4f20\u80cc\u666f\u3002 \u5728 C57BL\/6J \u996e\u98df\u8bf1\u5bfc\u80a5\u80d6\u6a21\u578b\u4e2d\uff0c\u6211\u4eec\u901a\u5e38\u4f7f\u7528 2 U\/kg \u7684\u80f0\u5c9b\u7d20\u8fdb\u884c\u6ce8\u5c04\u3002 \u6211\u4eec\u901a\u5e38\u5728\u7a7a\u8179\u72b6\u6001\u4e0b\u8fdb\u884c\u80f0\u5c9b\u7d20\u8010\u91cf\u8bd5\u9a8c\u3002 \u4f46\u662f\uff0c\u5982\u679c\u8003\u8651\u4e0d\u540c\u6a21\u578b\u7684\u76ee\u6807\u5206\u5b50\uff0c\u6709\u65f6\u53ef\u4ee5\u5728\u975e\u7981\u98df\u6761\u4ef6\u4e0b\u8fdb\u884c\u3002<\/p>\n\n\n\n

1\u3001\u6267\u884c\u65e9\u4e0a 7:00 \u5230\u4e0b\u5348 15:00 \u7684\u7981\u98df\u3002<\/p>\n\n\n\n

2\u3001\u6d4b\u91cf\u5c0f\u9f20\u7684\u4f53\u91cd\u3002<\/p>\n\n\n\n

3\u3001\u51c6\u5907\u80f0\u5c9b\u7d20\u548c\u8461\u8404\u7cd6\u3002 \u7528\u51b0\u9547\u7684\u65e0\u83cc PBS \u5c06\u80f0\u5c9b\u7d20\u7a00\u91ca\u6210 2 U\/10 ml\u3002\u7528\u80f0\u5c9b\u7d20\u9884\u88c5\u6ce8\u5c04\u5668\u5e76\u4fdd\u6301\u5728\u51b0\u4e0a\u3002 \u51c6\u5907\u4e00\u79cd\u6216\u4e24\u79cd\u8461\u8404\u7cd6\u65e0\u83cc\u6eb6\u6db2\uff0c\u4ee5\u9632\u6b62\u5c0f\u9f20\u4f4e\u8840\u7cd6\u3002<\/p>\n\n\n\n

4\u3001\u5728\u80f0\u5c9b\u7d20\u6ce8\u5c04\u540e 0\u300115\u300130\u300160\u300190 \u548c 120 \u5206\u949f\u91c7\u8840\u3002<\/p>\n\n\n\n

5\u3001\u6d4b\u91cf\u8461\u8404\u7cd6\u6c34\u5e73\u5e76\u89c2\u5bdf\u5c0f\u9f20\u3002 \u5982\u679c\u8001\u9f20\u751f\u7406\u72b6\u6001\u4e0d\u597d\uff0c\u7acb\u5373\u6ce8\u5c04\u8461\u8404\u7cd6\u4ee5\u907f\u514d\u6b7b\u4ea1\u3002<\/p>\n\n\n\n

3.9.\u8eab\u4f53\u6210\u5206\u6d4b\u91cf<\/strong><\/p>\n\n\n\n

\u968f\u7740\u6280\u672f\u7684\u8fdb\u6b65\uff0c\u78c1\u5171\u632f\u6210\u50cf (MRI) \u548c\u53cc\u80fd X \u5c04\u7ebf\u9aa8\u5bc6\u5ea6\u626b\u63cf (DEXA) \u9010\u6e10\u53d6\u4ee3\u4e86\u7528\u4e8e\u6d4b\u91cf\u8102\u80aa\u6210\u5206\u7684\u5316\u5b66\u80f4\u4f53\u5206\u6790\u3002\u5316\u5b66\u80f4\u4f53\u66fe\u7ecf\u88ab\u8ba4\u4e3a\u662f\u5168\u8eab\u6210\u5206\u5206\u6790\u7684\u9ec4\u91d1\u6807\u51c6\u3002\u4f46\u5b83\u9700\u8981\u727a\u7272\u8001\u9f20\u548c\u8d39\u65f6\u3002\u5bf9\u5355\u4e2a\u5c0f\u9f20\u7684\u8102\u80aa\u7ec4\u7ec7\u8fdb\u884c\u89e3\u5256\u548c\u79f0\u91cd\uff0c\u4e5f\u53ef\u4ee5\u6d4b\u91cf\u5168\u8eab\u8102\u80aa\u3002\u4f46\u662f\uff0c\u8fd9\u79cd\u65b9\u6cd5\u4e0d\u592a\u51c6\u786e\u3002\u5185\u810f\u8102\u80aa\u7684\u6536\u96c6\u53ef\u80fd\u5177\u6709\u6311\u6218\u6027\uff0c\u56e0\u4e3a\u5b83\u901a\u5e38\u96c6\u6210\u5728\u5185\u90e8\u5668\u5b98\u4e2d\uff0c\u65e0\u6cd5\u51c6\u786e\u89e3\u5256\u3002DEXA \u7684\u8eab\u4f53\u6210\u5206\u6d4b\u91cf\u7531\u4e09\u4e2a\u90e8\u5206\u7ec4\u6210\uff1a\u8102\u80aa\u3001\u9aa8\u77ff\u7269\u8d28\u548c\u7626\u8f6f\u7ec4\u7ec7\u3002\u4e0e\u5316\u5b66\u63d0\u53d6\u65b9\u6cd5\u76f8\u6bd4\uff0c\u5b83\u663e\u793a\u51fa\u8102\u80aa\u9762\u79ef\u767e\u5206\u6bd4\u4e0e\u5168\u8eab\u8102\u80aa\u4e4b\u95f4\u7684\u5f3a\u76f8\u5173\u6027\u3002\u65e0\u9700\u727a\u7272\u5c0f\u9f20\uff0c\u53ef\u7528\u4e8e\u8fde\u7eed\u6d4b\u91cf\u3002<\/p>\n\n\n\n

1\u3001\u6d4b\u91cf\u52a8\u7269\u4f53\u91cd\u5e76\u7528\u6c2f\u80fa\u916e\/\u7532\u82ef\u567b\u55ea\u6eb6\u6db2\u5c06\u5c0f\u9f20\u8179\u819c\u5185\u9ebb\u9189\u3002<\/p>\n\n\n\n

2\u3001\u4f7f\u7528\u5c0f\u9f20\u6a21\u578b\u6821\u51c6\u9aa8\u5bc6\u5ea6\u548c\u8102\u80aa\u542b\u91cf\u767e\u5206\u6bd4\u3002<\/p>\n\n\n\n

3\u3001\u5c06\u5c0f\u9f20\u8179\u90e8\u671d\u4e0b\u653e\u5728\u4e00\u6b21\u6027\u5851\u6599\u6258\u76d8\u4e0a\uff0c\u4ee5\u4fbf\u6d4b\u91cf\u6574\u4e2a\u5c0f\u9f20\u3002<\/p>\n\n\n\n

4\u3001\u8bb0\u5f55\u9aa8\u77ff\u7269\u8d28\u5bc6\u5ea6\u3001\u9aa8\u77ff\u7269\u8d28\u542b\u91cf\u3001\u7626\u8089\u91cf\u3001\u8102\u80aa\u91cf\u548c\u4f53\u8102\u767e\u5206\u6bd4\u3002<\/p>\n\n\n\n

5\u3001\u4f7f\u7528 PIXImus2 \u8f6f\u4ef6\u5206\u6790\u6570\u636e\u3002<\/p>\n\n\n\n

3.10 \u5c0f\u9f20\u96f7\u5e15\u9709\u7d20\u6cbb\u7597<\/strong><\/p>\n\n\n\n

1\u3001\u5c06 1 mg \u96f7\u5e15\u9709\u7d20\u6eb6\u89e3\u5728 20 \u03bcl \u4e59\u9187\u4e2d\u4f5c\u4e3a\u53e0\u52a0\u6eb6\u6db2\u3002<\/p>\n\n\n\n

2\u3001\u4e34\u7528\u524d\u7528\u6797\u683c\u6c0f\u76d0\u6eb6\u6db2\u8fdb\u4e00\u6b65\u7a00\u91ca\u6eb6\u89e3\u7684\u96f7\u5e15\u9709\u7d20\u81f3\u7ec8\u6d53\u5ea6\u4e3a 1 mg\/ml\u3002<\/p>\n\n\n\n

3\u3001\u7ea6\u675f\u8001\u9f20\uff0c\u9732\u51fa\u8179\u90e8\u3002<\/p>\n\n\n\n

4\u3001\u51c6\u5907 25 \u53f7\u9488\u5934\u5e76\u7528\u7a00\u91ca\u7684\u96f7\u5e15\u9709\u7d20\u586b\u5145\u6ce8\u5c04\u5668\u3002<\/p>\n\n\n\n

5\u3001\u5bf9\u6ce8\u5c04\u90e8\u4f4d\u8fdb\u884c\u6d88\u6bd2\uff0c\u5e76\u5c06\u9488\u5934\u4ee5 30\u00b0 \u89d2\u5411\u5934\u90e8\u63d2\u5165\uff0c\u4f4d\u4e8e\u8110\u5c3e\u4fa7\u548c\u4e2d\u7ebf\u5916\u4fa7\u3002<\/p>\n\n\n\n

6\u3001\u6bcf\u6b21\u6ce8\u5c04\u524d\uff0c\u8bf7\u52a1\u5fc5\u5728\u6ce8\u5c04\u524d\u8fdb\u884c\u62bd\u5438\uff0c\u4ee5\u514d\u6e17\u5165\u80a0\u9053\u6216\u80c6\u56ca\u3002<\/p>\n\n\n\n

3.11 \u5c0f\u9f20\u5904\u6b7b\u3001\u7ec4\u7ec7\u548c\u8840\u6db2\u7684\u6536\u96c6<\/strong><\/p>\n\n\n\n

\u5728\u996e\u98df\u5582\u517b\u5b9e\u9a8c\u4e4b\u540e\uff0c\u5904\u6b7b\u5c0f\u9f20\u4ee5\u6536\u96c6\u5b83\u4eec\u7684\u8840\u6db2\u3001\u5185\u810f\u5e76\u5206\u6790\u5168\u8eab\u6210\u5206\u3002\u7ec4\u7ec7\u4e5f\u5c06\u7528\u4e8e\u8fdb\u4e00\u6b65\u5206\u6790\uff0c\u5305\u62ec\u86cb\u767d\u8d28\u6216 RNA \u6216\u7ec4\u7ec7\u5b66\u5207\u7247\u3002<\/p>\n\n\n\n

\u4e0e\u5bf9\u7167\u5c0f\u9f20\u76f8\u6bd4\uff0c\u996e\u98df\u8bf1\u5bfc\u7684\u80a5\u80d6\u6a21\u578b\u4f1a\u5bfc\u81f4\u591a\u5668\u5b98\u53d8\u5316\u3002\u9ad8\u8102\u996e\u98df\u4f1a\u5bfc\u81f4\u809d\u810f\u8102\u80aa\u53d8\u6027\uff0c\u89e3\u5256\u540e\u53ef\u4ee5\u660e\u663e\u89c2\u5bdf\u5230\u8102\u80aa\u809d\u7684\u53d8\u5316\u3002\u8102\u80aa\u7ec6\u80de\u6570\u91cf\u548c\u5927\u5c0f\u589e\u52a0\u3002\u80f0\u5c9b\u4f1a\u53d8\u5f97\u80a5\u5927\uff0c\u7136\u540e\u9000\u5316\u3002\u9aa8\u9abc\u808c\u53ef\u80fd\u964d\u4f4e\u4e86\u80f0\u5c9b\u7d20\u654f\u611f\u6027\u3002\u68d5\u8272\u8102\u80aa\u53ef\u80fd\u4f1a\u51fa\u73b0\u840e\u7f29\u6216\u767d\u8272\u8102\u80aa\u6c89\u79ef\u3002\u56e0\u6b64\uff0c\u80a5\u80d6\u662f\u4e00\u79cd\u5168\u8eab\u7cfb\u7edf\u7d0a\u4e71\u3002\u5c3d\u7ba1\u67d0\u4e9b\u5668\u5b98\u7cfb\u7edf\u6bd4\u5176\u4ed6\u7cfb\u7edf\u53d7\u5230\u7684\u5f71\u54cd\u66f4\u5927\uff0c\u4f46\u6211\u4eec\u901a\u5e38\u4f1a\u6309\u987a\u5e8f\u6536\u96c6\u6d89\u53ca\u7684\u5668\u5b98\u4ee5\u907f\u514d\u6570\u636e\u4e22\u5931\u3002<\/p>\n\n\n\n

1\u3001\u5904\u6b7b\u524d\u51c6\u5907\u597d\u5904\u6b7b\u677f\u3001\u6ce8\u5c04\u5668\u3001eppendorf \u7ba1\u3001\u89e3\u5256\u5de5\u5177\u3001\u704c\u6ce8\u5de5\u5177\u548c\u51b0\u9547 PBS \u6eb6\u6db2\u3002<\/p>\n\n\n\n

2\u3001\u6807\u8bb0\u6bcf\u4e2a eppendorf \u7ba1\u4ee5\u4f9b\u8840\u6e05\u3001\u7ec4\u7ec7\u6536\u96c6\u6216\u7ec4\u7ec7\u5b66\u56fa\u5b9a\u3002\u727a\u7272\u524d\u586b\u5145 3.7% \u7532\u919b\u6216\u7ec4\u7ec7\u88c2\u89e3\u7f13\u51b2\u6db2\u6216 RNA \u63d0\u53d6\u6eb6\u6db2 (Trizol)\u3002<\/p>\n\n\n\n

3\u3001\u6839\u636e\u52a8\u7269\u89c4\u7a0b\u548c\u4f26\u7406\u89c4\u5b9a\uff0c\u7528 CO2 \u5bf9\u5c0f\u9f20\u5b9e\u65bd\u5b89\u4e50\u6b7b\u3002<\/p>\n\n\n\n

4\u3001\u7528\u9488\u5c06\u5c0f\u9f20\u7684\u56db\u80a2\u56fa\u5b9a\u5728\u89e3\u5256\u677f\u4e0a\u3002<\/p>\n\n\n\n

5\u3001\u5728\u8110\u90e8\u9644\u8fd1\u6c34\u5e73\u5207\u5f00\u76ae\u80a4\u3002
6\u3001\u53bb\u9664\u76ae\u80a4\u548c\u76ae\u4e0b\u5c42\u3002<\/p>\n\n\n\n

7\u3001\u901a\u8fc7\u8179\u90e8\u4e2d\u7ebf\u505a\u4e00\u4e2a\u5782\u76f4\u5207\u53e3\uff0c\u6253\u5f00\u8179\u90e8\u3002<\/p>\n\n\n\n

8\u3001\u5207\u5f00\u80f8\u8154\uff0c\u9732\u51fa\u5fc3\u810f\u548c\u80ba\u3002<\/p>\n\n\n\n

9\u3001\u5c06\u9488\u5934\u63d2\u5165\u5fc3\u810f\u5de6\u5fc3\u5ba4\u5fc3\u5c16\u90e8\uff0c\u7f13\u6162\u62bd\u8840\uff0c\u907f\u514d\u8fc7\u65e9\u62bd\u8840\u3002\u4e00\u53ea\u5c0f\u9f20\u53ef\u91c7\u96c6 300\u20131,000 \u03bcl \u8840\u6db2\u3002<\/p>\n\n\n\n

10\u3001\u5c06\u8840\u6db2\u6536\u96c6\u5230\u91c7\u8840 eppendorf \u7ba1\u4e2d\u3002<\/p>\n\n\n\n

11\u3001\u5148\u6536\u96c6\u80f0\u817a\uff0c\u56e0\u4e3a\u5b83\u4eec\u7684 RNA \u548c\u86cb\u767d\u8d28\u66f4\u5bb9\u6613\u88ab\u80f0\u9176\u7834\u574f\u3002<\/p>\n\n\n\n

12\u3001\u5982\u679c\u9700\u8981\uff0c\u6536\u96c6\u5fc3\u3001\u80ba\u3001\u809d\u3001\u813e\u3001\u80be\u548c\u80a0\u3002<\/p>\n\n\n\n

13\u3001\u6536\u96c6\u76ae\u4e0b\u3001\u8179\u80a1\u6c9f\u548c\u80a0\u7cfb\u819c\u767d\u8272\u8102\u80aa\u57ab\u3002<\/p>\n\n\n\n

14\u3001\u6536\u96c6\u80cc\u90e8\u7684\u68d5\u8272\u8102\u80aa\u3002<\/p>\n\n\n\n

15\u3001\u5982\u679c\u9700\u8981\uff0c\u6536\u96c6\u5927\u8111\u3002<\/p>\n\n\n\n

16\u3001\u8fdb\u884c\u7ec4\u7ec7\u5b66\u6216\u7ec4\u7ec7\u5206\u6790\uff08\u89c1\u6ce8 7\uff09\u3002<\/p>\n\n\n\n

\u6ce87 \u5bf9\u4e8e\u767d\u8272\u8102\u80aa\u7ec4\u7ec7\u4e0e\u7ec4\u7ec7\u5207\u7247\u7684\u6bd4\u8f83\uff0c\u9700\u8981\u5c06\u767d\u8272\u8102\u80aa\u7684\u591a\u4e2a\u4f4d\u7f6e\u4e0e\u8fde\u7eed\u5207\u7247\u8fdb\u884c 20 \u591a\u5f20\u5207\u7247\u8fdb\u884c\u6bd4\u8f83\uff0c\u4ee5\u83b7\u5f97\u51c6\u786e\u7684\u6570\u636e\u3002 \u6211\u4eec\u901a\u5e38\u6536\u96c6\u68d5\u8272\u8102\u80aa\u9644\u8fd1\u7684\u76ae\u4e0b\u8102\u80aa\u3001\u8179\u80a1\u6c9f\u8102\u80aa\u3001\u80a0\u7cfb\u819c\u8102\u80aa\u3001\u6027\u817a\u8102\u80aa\u548c\u767d\u8272\u8102\u80aa\uff0c\u4ee5\u5168\u9762\u4e86\u89e3\u5c0f\u9f20\u4e4b\u95f4\u7684\u767d\u8272\u8102\u80aa\u5dee\u5f02\u3002<\/p>\n\n\n\n

\u539f\u6587\u4e0b\u8f7d\uff1a<\/strong>A Mouse Model of Diet-Induced Obesity and Insulin Resistance (2014 downloads )<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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