For sixteen years, the American Institute of Nutrition Rodent Diets, AIN-76 and AIN-76A, have been used extensively around the world. Because of numerous nutritional and technical problems encountered with the diet during this period, it was revised.<\/p>\n\n\n\n
Two new formulations were derived: AIN-93G for growth, pregnancy and lactation, and AIN-93M for adult main tenance.<\/p>\n\n\n\n
Some major differences in the new formulation of AIN-93G compared with AIN-76A are as follows:<\/p>\n\n\n\n
7 g soybean oil\/100 g diet was substituted for 5 g corn oil\/100 g diet to increase the amount of linolenic acid;<\/p>\n\n\n\n
cornstarch was substituted for sucrose;<\/p>\n\n\n\n
the amount of phosphorus was reduced to help eliminate the problem of kidney calcification in female rats;<\/p>\n\n\n\n
L-cystine was substituted for DL-methionine as the amino acid supplement for casein, known to be deficient in the sulfur amino acids;<\/p>\n\n\n\n
manganese concentration was lowered to onefifth the amount in the old diet;<\/p>\n\n\n\n
the amounts of vitamin E, vitamin K and vitamin B-12 were increased;<\/p>\n\n\n\n
and molybdenum, silicon, fluoride, nickel, boron, lithium and vanadium were added to the mineral mix.<\/p>\n\n\n\n
For the AIN-93M maintenance diet, the amount of fat was lowered to 40 g\/kg diet from 70 g\/kg diet, and the amount of casein to 140 g\/kg from 200 g\/kg in the AIN-93G diet.<\/p>\n\n\n\n
Because of a better balance of essential nutrients, the AIN-93 diets may prove to be a better choice than AIN-76A for long-term as well as short-term studies with laboratory rodents.<\/p>\n\n\n\n
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