Ain93系列配方替代Ain76A饮食的说明

原文摘要：

For sixteen years, the American Institute of Nutrition Rodent Diets, AIN-76 and AIN-76A, have been used extensively around the world. Because of numerous nutritional and technical problems encountered with the diet during this period, it was revised.

Two new formulations were derived: AIN-93G for growth, pregnancy and lactation, and AIN-93M for adult main tenance.

Some major differences in the new formulation of AIN-93G compared with AIN-76A are as follows:

7 g soybean oil/100 g diet was substituted for 5 g corn oil/100 g diet to increase the amount of linolenic acid;

cornstarch was substituted for sucrose;

the amount of phosphorus was reduced to help eliminate the problem of kidney calcification in female rats;

L-cystine was substituted for DL-methionine as the amino acid supplement for casein, known to be deficient in the sulfur amino acids;

manganese concentration was lowered to onefifth the amount in the old diet;

the amounts of vitamin E, vitamin K and vitamin B-12 were increased;

and molybdenum, silicon, fluoride, nickel, boron, lithium and vanadium were added to the mineral mix.

For the AIN-93M maintenance diet, the amount of fat was lowered to 40 g/kg diet from 70 g/kg diet, and the amount of casein to 140 g/kg from 200 g/kg in the AIN-93G diet.

Because of a better balance of essential nutrients, the AIN-93 diets may prove to be a better choice than AIN-76A for long-term as well as short-term studies with laboratory rodents.

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译文：

16年来，美国营养研究所的啮齿动物饮食AIN-76和AIN-76A已在世界各地广泛使用。

由于在此期间饮食遇到了许多营养和技术问题，因此对其进行了修订。

由此开发了两个新配方：AIN-93G用于生长、妊娠和哺乳，AIN-93M用于成年期维持。

与AIN-76A相比，AIN-93G新配方的一些主要区别如下：

用7克大豆油/100克日粮代替5克玉米油/100克日粮，以增加亚麻酸的量；

用玉米淀粉代替蔗糖；

减少磷的量，以帮助消除雌性大鼠肾脏钙化的问题；

L-胱氨酸代替DL-蛋氨酸作为酪蛋白的氨基酸补充剂，因为酪蛋白中缺乏含硫氨基酸；

锰浓度降低到原来饮食的五分之一；

维生素E、维生素K、维生素B-12含量增加；

并且将钼、硅、氟化物、镍、硼、锂和钒加入到矿物混合物中。

对于AIN-93M维持日粮，脂肪的量从70g/kg日粮降低到40g/kg日粮，并且在AIN-93G日粮中酪蛋白的量从200g/kg降低到140g/kg.

由于必需营养素的更好平衡，AIN-93饮食因此可能被证明是比AIN-76A更好的进行实验室啮齿类动物研究的饮食选择。

阅读更多原文：实验室啮齿动物的AIN-93纯化饮食：美国营养学会特设写作委员会关于AIN-76A啮齿动物饮食重新制定的最终报告 – PubMed (nih.gov)